Avocado Seed Extracts: Research Summary

## Research summary:
A 2022 review published in Science Direct}} examined the chemical makeup and biological properties of avocado seed extracts. Here’s what the research found:

### Chemical Composition
Avocado seeds contain concentrated amounts of bioactive compounds including catechin, epicatechin, procyanidins, acetogenins, flavonoids, triterpenes, phytosterols, and polyhydroxylated fatty alcohols.

### Laboratory and Animal Studies

In test-tube studies and animal models, these seed extracts showed several biological activities:
* Anti-inflammatory effects by suppressing inflammatory markers like TNF-α, IL-1β, and nitric oxide
* Strong antioxidant activity, neutralizing reactive oxygen species and stabilizing free radicals
* Blood sugar and cholesterol reduction in diabetic or high-cholesterol rats, possibly by promoting glycogen storage and affecting liver metabolism
* Inhibition of cholinesterase enzymes (AChE and BChE), suggesting potential cognitive support
* Induced cell death and inhibited growth in several cancer cell lines (breast, colon, liver, lung) through polyphenol and triterpenoid mechanisms

### Limitations
All these findings come from preclinical research. There are no confirmed human clinical trials demonstrating safety or effectiveness for topical or oral use. The authors conclude that while avocado seeds show promising pharmacological potential and could be developed as functional ingredients, further toxicological testing and controlled human studies are needed before any home or medical use can be considered evidence-based.

### Reference
Comprehensive review article:
Tremocoldi MA, Rosalen PL, Franchin M, et al. Avocado seed discoveries: Chemical composition, biological properties, and industrial food applications. Food Chem X. 2022;16:100525. https://www.sciencedirect.com/science/article/pii/S2590157522003054

Comprehensive Tincture ABV Reference – pdf

The comprehensive tincture ABV reference is available as a downloadable PDF for offline use. This 22-page document contains alcohol percentages and extraction ratios for 300+ herbs. For the searchable online version, see [Herbal Tincture ABV Reference Guide](https://dittany.com/abv_reference/)

[![Herbal_Tincture_ABV_Reference — Comprehensive tincture ABV reference](/wp-content/uploads/Herbal_Tincture_ABV_Reference-pdf-300×232.jpg){.alignnone}](/wp-content/uploads/Herbal_Tincture_ABV_Reference.pdf)

Herbal Tincture ABV Reference Guide

Whether you’re a home herbalist or clinical practitioner, getting the alcohol percentage right is crucial for both effective extraction and long-term preservation of your tinctures. This comprehensive reference combines field-tested data from two respected sources to help you make informed decisions about your herbal preparations.
## How to Use This Reference
Simply type any herb name in the search box to instantly find:
*** Recommended alcohol percentages
* Fresh vs. dry preparation methods
* Weight-to-volume ratios
* Water and glycerin percentages where applicable
* Important safety notes**
### Understanding the Data
This reference synthesizes:
Pacific Northwest field data from Scott Kloos (2010) – tested ratios for fresh and dry preparations
General extraction guidelines from Nikki Hess/Monarch Herbs – broader ABV ranges for dry herbs
Where sources differ, both are listed so you can make informed choices based on your materials and methods.
### Key Principles to Remember
#### Alcohol Percentage Basics:
High alcohol (70-95%): For resins, volatile oils, and alkaloids (e.g., propolis, myrrh, fresh aromatic herbs)
Medium alcohol (40-70%): For most standard extractions, balanced constituents
Low alcohol (20-40%): For water-soluble constituents like polysaccharides, minerals, and mucilage
#### Fresh vs. Dry Considerations:
Fresh plants contain significant water content, so they typically require higher alcohol percentages to achieve the desired final concentration. Dry herbs have moisture removed, allowing lower initial alcohol percentages.
#### Preservation Note:
While some references show alcohol as low as 20-25%, this is the absolute minimum for preservation. For long-term storage (5+ years), 30% or higher is recommended. Remember that alcohol evaporates faster than water, so percentages decrease over time.
#### *Safety First*
Several entries include important safety notes – particularly for toxic plants (like Yew), photosensitizing herbs (Cow Parsnip), or those requiring special preparation (Cascara Sagrada must age one year). Always research thoroughly before working with unfamiliar plants.

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### About This Project
This reference was compiled to make tincture-making information more accessible to the herbal community. The data represents decades of combined experience from respected herbalists and should serve as a starting point for your own practice.
For educational purposes only. Always verify plant identification and safety before use. Consult qualified practitioners for medical advice.
#### Sources:
**Pacific Northwest Tincture Chart** © 2010 Scott Kloos
**Herbal Tincture ABV Reference** compiled by Nikki Hess, Monarch Herbs

Test Sunscreen at Home: Step-by-Step Methods

## Test Your Sunscreen at Home: Step-by-Step Methods

When 16 out of 20 commercial sunscreens failed their SPF claims in Australian testing, consumers lost a reliable way to trust product labels. Professional sunscreen testing requires specialized laboratories, but you can assess UV protection effectiveness using simple materials and clear procedures.

## Method 1: UV Bead Testing

**Materials Needed:**
– UV-sensitive beads (available from educational science suppliers like Steve Spangler Science or Carolina Biological)
– 4-6 clear plastic sandwich bags
– Permanent marker
– Various sunscreen products to test
– Timer or watch
– Notebook for recording results

**Step-by-Step Instructions:**

1. **Prepare Your Test Setup**
– Work in shade initially to prevent premature bead activation
– Count out equal portions of UV beads (15-20 beads per test)
– Place one portion in each plastic bag

2. **Label Your Samples**
– Mark one bag “Control” (no sunscreen)
– Label remaining bags with product names and SPF numbers
– Write labels on the side that will face down during testing

3. **Apply Sunscreen**
– Spread a thin, even layer of sunscreen on the unmarked side of each bag
– Use approximately half a teaspoon per bag
– Leave the control bag untreated
– Allow sunscreen to dry completely (5-10 minutes)

4. **Conduct the Test**
– Place all bags label-side down in direct sunlight
– Start your timer
– Check bags every 10 minutes for the first hour
– Record color changes in your notebook

5. **Evaluate Results**
– Arrange bags from brightest colors (least protection) to palest colors (best protection)
– Compare each sunscreen’s performance to your control sample
– Document which products kept beads closest to white

**What to Expect:**
UV beads will always show some color change, even under effective sunscreen. Look for relative differences rather than complete color blocking.

## Method 2: Construction Paper Testing

**Materials Needed:**
– Black construction paper
– Clear plastic wrap
– Tape
– Scissors
– Various sunscreen products
– Coins or small weights

**Step-by-Step Instructions:**

1. **Prepare Paper Samples**
– Cut black construction paper into 3×3 inch squares
– Cut one square for each sunscreen plus one control square

2. **Apply Sunscreen**
– Leave one square untreated as your control
– Apply a thin layer of each sunscreen to separate squares
– Rub sunscreen into paper surface until absorbed
– Label each square clearly

3. **Set Up Sun Exposure**
– Place squares in direct sunlight
– Weight down corners with coins to prevent movement
– Expose for 4-6 hours during peak sun (10 AM – 4 PM)

4. **Assess Fading**
– Compare treated squares to your untreated control
– Less fading indicates better UV protection
– Document differences photographically if possible

## Method 3: Comparative Analysis

**Materials Needed:**
– Your testing material of choice (UV beads or construction paper)
– Commercial sunscreen with known SPF rating
– Products you want to test

**Step-by-Step Instructions:**

1. **Establish Baseline**
– Test a commercial sunscreen with verified SPF rating
– Use this as your reference point for comparison

2. **Test Unknown Products**
– Apply same testing method to products with unknown effectiveness
– Compare results directly to your baseline product

3. **Rate Effectiveness**
– If your baseline SPF 30 product keeps beads pale yellow, products producing similar colors offer comparable protection
– Products showing brighter colors provide less protection
– Products showing paler colors may offer better protection

## Recording and Interpreting Results

**Documentation Method:**
Create a simple chart with columns for:
– Product name and claimed SPF
– Time intervals (10 min, 20 min, 30 min, 1 hour)
– Color intensity (scale 1-5, with 1 being white and 5 being brightest color)
– Weather conditions and UV index if available

**Understanding Your Results:**
– Effective products will show minimal color change in UV beads or less fading in paper
– Products performing significantly worse than their SPF claims need replacement
– Consistent poor performance across multiple tests indicates unreliable protection

**Important Testing Conditions:**
– Test during peak UV hours (10 AM – 4 PM)
– Use direct sunlight, not shade or indoor lighting
– Maintain consistent application thickness across all samples
– Record weather conditions and time of day

## Safety and Limitations

**Testing Safety:**
– Never expose your own skin during testing
– Work quickly when setting up tests in direct sun
– Wear protective clothing and sunscreen while conducting tests

**Understanding Limitations:**
Home testing provides comparative data rather than absolute SPF measurements. Professional testing accounts for factors like skin absorption, water resistance, and precise UV measurement that home methods cannot replicate.

Use these results to identify products that perform significantly better or worse than expected, not to determine exact SPF values.

**When to Replace Products:**
– Products showing poor performance in multiple tests
– Sunscreens older than two years
– Products exposed to high heat or contamination
– Any product that has caused unexpected sunburn despite proper application

These testing methods empower you to verify sun protection effectiveness when commercial testing has proven unreliable. Regular home testing provides ongoing quality control for the products protecting your skin.

## Sources and Further Reading:
Steve Spangler Science – UV Color-Changing Beads https://www.stevespanglerscience.com/store/uv-color-changing-beads.html

Science Buddies – Testing Sunscreen Effectiveness and Water Solubility https://www.sciencebuddies.org/science-fair-projects/project-ideas/GreenChem_p007/green-chemistry/sunscreen-effectiveness-water-solubility

TeachEngineering – How Effective Is Your Sunscreen Activity https://www.teachengineering.org/activities/view/van_nanoparticles_lesson02_activity2

FDA – Labeling and Effectiveness Testing: Sunscreen Drug Products https://www.fda.gov/regulatory-information/search-fda-guidance-documents/labeling-and-effectiveness-testing-sunscreen-drug-products-over-counter-human-use-small-entity

BBC News – The Sunscreen Scandal Shocking Australia https://www.bbc.com/news/articles/c4gzl41rpdqo